Électroporation

Électroporation

L'électroporation est une méthode d'introduction d'ADN dans des cellules. Techniquement, on applique un champs électrique sur les membranes qui sont ainsi déstabilisées, et l'ADN présent dans l'espace extra-cellulaire peut rentrer dans les cellules en migrant vers le pôle positif de la charge, étant lui-même chargé négativement.

Voir aussi


  • Portail de la biologie cellulaire et moléculaire Portail de la biologie cellulaire et moléculaire

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  • Electroporation — Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way… …   Wikipedia

  • electroporation — noun Etymology: electr + 2pore + ation Date: 1985 the application of an electric current to a living surface (as the skin or a cell membrane) in order to open pores or channels through which something (as a drug or DNA) may pass …   New Collegiate Dictionary

  • electroporation — (e lek tro pě ra shěn) The application of an electric field to create temporary pores in the plasma membrane in order to insert DNA into the cell and transform it …   Dictionary of microbiology

  • electroporation — Method for temporarily permeabilising cell membranes so as to facilitate the entry of large or hydrophilic molecules (as in transfection). A brief (ca 1 msec) electric pulse is given with potential gradients of about 700V/cm …   Dictionary of molecular biology

  • electroporation — noun ɪˌlɛktɹoʊpɔˈɹeɪʃən The creation of transient pores in the cell membrane, through the application of a high voltage electrical pulse. Syn: electropermeabilization See Also: electroporate, electroporator …   Wiktionary

  • electroporation — A technique in which a brief electric shock is applied to cells; momentary holes open briefly in the plasma membrane, allowing the entry of macromolecules ( e.g., a way of introducing new DNA into a cell). * * * elec·tro·po·ra·tion i .lek trə… …   Medical dictionary

  • electroporation — [ɪˌlɛktrə(ʊ)pə reɪʃ(ə)n] noun Biology the process of introducing DNA or chromosomes into cells using an electrical pulse to create temporary pores in the cell membranes. Derivatives electroporate verb …   English new terms dictionary

  • electroporation — elec·tro·po·ra·tion …   English syllables

  • electroporation — The induction of transient pores in bacterial cells or protoplasts by the application of a pulse of electricity. These pores allow the entry of exogenous DNA into the cell. Widely used for the transformation of bacteria …   Glossary of Biotechnology

  • électroporation —  n.f. Technique de manipulation génétique …   Le dictionnaire des mots absents des autres dictionnaires

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